US 17, FL 31
195.4 mi3 hours 34 mins
| Head east on NASA Parkway West (FL 405) | 700 feet | |
| Make a U-turn and continue on NASA Parkway West (FL 405) | 4.3 mi | |
| Continue onto NASA Causeway (FL 405) | 2.5 mi | |
| Continue onto Columbia Boulevard (FL 405) | 1.7 mi | |
| Turn left onto Challenger Memorial Parkway (FL 407) | 6.8 mi | |
| Merge left onto Beachline Expressway (FL 528 Toll W) | 20.6 mi | |
| Take the ramp towards FL 417: Orlando | 0.3 mi | |
| Keep left towards FL 417 South: Tampa | 1.3 mi | |
| Merge left onto Central Florida GreeneWay (FL 417 Toll S) | 17.1 mi | |
| Keep left onto Central Florida GreeneWay (FL 417 Toll S) | 1.2 mi | |
| Continue onto Southern Connector Extension (FL 417 Toll S) | 7.0 mi | |
| Keep left towards Celebration | 0.4 mi | |
| Keep left towards I 4 West: Tampa | 0.8 mi | |
| Merge left onto I 4 W | 13.0 mi | |
| Take the ramp towards CR 557: Lake Alfred | 900 feet | |
| Turn right onto Old Grade Road (CR 557) | 6.4 mi | |
| Continue onto Pomelo Street (CR 557) | 400 feet | |
| Turn right onto Shinn Boulevard (US 17) | 0.6 mi | |
| Continue onto Lake Shore Way (US 17) | 0.6 mi | |
| Go straight onto US 17 | 45.7 mi | |
| Continue onto US 17 | 18.3 mi | |
| Turn left onto Magnolia Street (FL 70) | 0.7 mi | |
| Continue onto Oak Street (FL 70) | 1.0 mi | |
| Turn right onto Hog Bay Avenue (FL 31) | 5.6 mi | |
| Turn right onto FL 31 | 30.8 mi | |
| Turn right onto Palm Beach Boulevard (FL 80) | 7.6 mi | |
| Turn left onto Fowler Street (US 41 Business) | 800 feet | |
| Turn right onto Second Street | 0.3 mi | |
| Turn left onto Hendry Street | 700 feet | |
| Turn right onto Dr Martin Luther King Boulevard (FL 82) | 100 feet | |
| You have arrived at your destination |
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Kennedy Space Center, Florida - Fort Myers, Florida