Highway 401, I 75 $south
416.3 mi6 hours 42 mins
| Head southwest | 200 feet | |
| Turn right | 200 feet | |
| Turn left onto White Oaks Boulevard | 400 feet | |
| Turn left onto Trafalgar Road (3) | 0.4 mi | |
| Take the ramp on the right towards QEW: Hamilton | 0.3 mi | |
| Merge left onto Queen Elizabeth Way (QEW S) | 10.6 mi | |
| Keep right towards 403: Hamilton (Downtown) | 51.7 mi | |
| Merge left onto Highway 401 (401 W) | 31.0 mi | |
| Keep left onto Highway 401 (401 W) | 105.9 mi | |
| Keep left onto Macdonald–Cartier Freeway (401 W) | 1.3 mi | |
| Continue onto Rt. Hon. Herb Gray Parkway (401 W) | 2.1 mi | |
| Take the ramp towards 3 West: LaSalle | 0.4 mi | |
| Merge left onto Talbot Road (3) | 0.3 mi | |
| Continue onto Huron Church Road (3) | 3.8 mi | |
| Take the ramp on the left towards Bridge to USA | 0.3 mi | |
| Continue slightly right onto Ambassador Bridge | 1.3 mi | |
| Keep left at the fork | 0.6 mi | |
| Merge left onto Fisher Freeway (I 75 S) | 13.0 mi | |
| Continue onto Detroit-Toledo Expressway (I 75 S) | 53.8 mi | |
| Keep left onto I 75 S | 138.2 mi | |
| Keep right towards SR 48 | 0.2 mi | |
| Turn left onto North Main Street (SR 48 S) | 0.5 mi | |
| Turn right onto West Monument Avenue (SR 4) | 500 feet | |
| Turn left onto North Ludlow Street (SR 48) | 0.3 mi | |
| Turn right onto West 3rd Street | 100 feet | |
| You have arrived at your destination |
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Oakville, Ontario - Dayton, Ohio